Specification
| Description | Recombinant protein from the full-length sequence of Homo sapiens melanocyte inducing transcription factor (MITF), transcript variant 4 (NM_000248). |
| Organism | Homo sapiens (Human) |
| Expression Host | Human Cells |
| Tag Info | His or DYKDDDDK. Please contact us if you need further information or require specific designed tag. |
| Purity | Greater than 90% by SDS-PAGE gel |
| Uniprot ID | O75030 |
| Entry Name | MITF_HUMAN |
| Gene Names | MITF BHLHE32 |
| Alternative Gene Names | BHLHE32 |
| Alternative Protein Names | Microphthalmia-associated transcription factor (Class E basic helix-loop-helix protein 32) (bHLHe32) |
| Application | Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies; For research & development use only! |
| Buffer | Purified protein formulated in a sterile solution of PBS buffer, pH7.2, without any preservatives |
| Endotoxin | Endotoxin level is < 0.1 ng/µg of protein (<1EU /µg) |
| Length | 526 |
| Molecular Weight(Da) | 58795 |
| Protein Sequence | (The sequence of expressed protein may have some variation from the sequence shown below. Please contact us for the exact sequence.) MQSESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSSSAEHPGASKPPISSSSMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVPVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC |
Background
| Function | FUNCTION: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. {ECO:0000269|PubMed:10587587, ECO:0000269|PubMed:22647378, ECO:0000269|PubMed:27889061, ECO:0000269|PubMed:9647758}. |
| Pathway | |
| Protein Families | MiT/TFE family |
| Tissue Specificity | Expressed in melanocytes (at protein level). {ECO:0000269|PubMed:28842328}.; TISSUE SPECIFICITY: [Isoform A2]: Expressed in the retinal pigment epithelium, brain, and placenta (PubMed:9647758). Expressed in the kidney (PubMed:9647758, PubMed:10578055). {ECO:0000269|PubMed:10578055, ECO:0000269|PubMed:9647758}.; TISSUE SPECIFICITY: [Isoform C2]: Expressed in the kidney and retinal pigment epithelium. {ECO:0000269|PubMed:10578055}.; TISSUE SPECIFICITY: [Isoform H1]: Expressed in the kidney. {ECO:0000269|PubMed:10578055, ECO:0000269|PubMed:9647758}.; TISSUE SPECIFICITY: [Isoform H2]: Expressed in the kidney. {ECO:0000269|PubMed:10578055, ECO:0000269|PubMed:9647758}.; TISSUE SPECIFICITY: [Isoform M1]: Expressed in melanocytes. {ECO:0000269|PubMed:20163701}.; TISSUE SPECIFICITY: [Isoform Mdel]: Expressed in melanocytes. {ECO:0000269|PubMed:20163701}. |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |